Back to: O level Biology NOTES Uganda syllabus
1.HAND LENS:
A normal hand lens is a convex lens mounted in a frame. It is placed a shorter distance of about 5cm from the eye and the object.
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Determination of magnification using a hand lens
Magnification refers to how much larger the object appears compared to its real size.
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Example
Calculate the magnification of an object, which is 10cm tall whose image appears to be 20cm tall.
Solution
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Magnification = 20/10
=x2
2.MICROSCOPES
There are two types of microscopes i.e.
- The electron microscope which uses a beam of electrons.
- The compound light microscope
THE COMPOUND LIGHT MICROSCOPE
It is called so because it uses a beam of light to view objects and has more than one convex lens. it is used in hospitals, schools and some industries.
Structure of a compound light microscope.
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FUNCTIONS OF THE DIFFERENT PARTS1.
1.Eye Piece:
Enables one to view the specimen
It magnifies the image from the objective lens.
2.Barrel:
Provides support for the eye piece and objective lens.
3.Nose piece/ turret/:
It holds the objective lenses in position
Can be rotated to position a particular lens required for a particular magnification.
4.Stage:
It is where a prepared slide is placed for observation.
5.Mirror:
It reflects light from external source through the specimen.
6.Stand / Base:
Supports instrument in on a flat surface.
7.Diaphragm:
Regulates the amount of light passing through the specimen.
8.Condenser:
Concentrates the light reflected by the mirror through the object / specimen on the stage.
9.Arm:
Used for carrying the instrument.
10.Clip:
Keeps the slide firmly on the stage.
11.Coarse adjustment knob:
Used for focusing of the object under study.
12.Fine adjustment knob:
Brings specimen into a sharp clearer focus (final focusing).
13.Objective lens:
Magnifies the specimen under study.
They are normally two or three. Low power (shortest), medium power and high power (longest).
Care of a microscope
The microscope is very delicate, expensive instrument which is very useful in the study of biology. Thus it should be handled carefully doing the following;
- It should be carried with both hands.Should never be dropped.
- Always kept in an upright position
- Only wipe the lens with soft lens tissue.
- It should always be kept in its special designed box.
Determination of magnification of a microscope.
Magnification refers to how much larger the object appears compared to its real size.
Magnification = magnification of the eye piece lens X magnification of the objective lens.
Example:
If the eye piece is marked x10 and the magnification of the objective lens is x40, what is the total magnification of a microscope?Magnification = magnification of the eye piece lens x magnification of the objective lens.
=10 x 40=400
The specimen was magnified x400
Let magnifying objective lens. (x4)